the effect of hyperthermia on survival fraction of du 145 human prostate carcinoma cell line in monolayer and spheroid culture
نویسندگان
چکیده
abstract background: hyperthermia (also called thermal therapy or thermotherapy) is a type of cancer treatment in which body tissue is exposed to high temperatures (up to 113°f). research has shown that high temperatures can damage and kill cancer cells, usually with minimal injury to normal tissues. for many years, biologists have investigated cancer by using monolayer cell culture. it is becoming more and more apparent that two-dimensional cell culture (monolayer) can not completely represent the real structure and characteristics of three-dimensional in vivo solid tumors. these spheroids resemble in vivo tumor models in several aspects. therefore, studying growth characteristics and behavior of spheroids is beneficial in understanding the behavior of tumors under various experimental conditions. methods: in this work we have studied and analyzed the in vitro response of human prostatic carcinoma cell line du 145 from monolayer and spheroid culture to hyperthermia. for this purpose the du 145 cells were cultured either as monolayer or spheroids. the thermal response was judged by the survival fraction of colony forming cells in spheroids or monolayer culture following heat treatment. results: the result of survival curves has shown that heating cells at 40°c and 41°c has no significant effect on cell viability and survival fraction at various times of heating but heating the cells at 42°c and 43°c in long period of heat treatment reduce the viability and survival fraction particularly. conclusion: heat shock at 44°c and 45°c has great effect on this cell viability and survival fraction but in any time and temperature, spheroids were more resistant than monolayer’s.
منابع مشابه
Comparison of radiosensitizing effect of Resveratrol on monolayer and spheroid culture of DU145 prostatic cell line
Background: Radiotherapy is an established therapeutic modality for prostate cancer. Resveratrol, a natural antioxidant, has been shown to inhibit carcinogenesis and to block the process of tumor initiation and progression. No data is available on the response of cellular spheroid to Reseveratol. In this study we have examined the effect of Resveratol on the radiation response of human p...
متن کاملthe effect of quercetin and hyperthermia on spheroid model of du145 prostate carcinoma cell line
the purpose of this study is to investigate the effects of quercetin as an inhibitor of heat shock proteins and hyperthermia on the induced dna damages and colony formation ability of du145 tumor spheroid culture. du145 cells were cultured as spheroids. on day11, spheroids with mean diameter 100 μm were treated with different concentration of quercetin for 24 hours and then exposed to hyperther...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
The role of Rad51 protein in radioresistance of spheroid model of DU145 prostate carcinoma cell line
Background: Rad51 is a protein with critical role in double strand break repair. Down-regulation of this protein has a significant effect in radiosensitivity of some cell lines like prostate carcinoma. Compared to monolayer cell culture model, the spheroids are more resistant to radiation. The aim of the current study was to determine the Rad51 protein level in DU145 spheroids, and monol...
متن کاملThe Effects of Extremely Low Frequency Pulsed Electromagnetic Field on Biochemical Properties of the Prostate Cancer Cell Line, DU-145
Background: In the recent decades, there has been an increasing effort to study possible biological effects of extremely low frequency electromagnetic fields (ELF-EMFs). In this study, the effects of 50 Hz, 0.6 mT pulsed electromagnetic field on proliferation and biochemical properties, in a prostate cancer cell line, DU-145, and the simultaneous treatment of these cells with electromagnetic fi...
متن کاملcomparison of radiosensitizing effect of resveratrol on monolayer and spheroid culture of du145 prostatic cell line
background: radiotherapy is an established therapeutic modality for prostate cancer. resveratrol, a natural antioxidant, has been shown to inhibit carcinogenesis and to block the process of tumor initiation and progression. no data is available on the response of cellular spheroid to reseveratol. in this study we have examined the effect of resveratol on the radiation response of human prostate...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of cancer preventionجلد ۲، شماره ۴، صفحات ۰-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023